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Natalie Elia: Unlocking the mechanism of the cellular ESCRT membrane fission machine by high-resolution microscopy

Joint seminar: Molecular Biology & Microbiology

2019.11.07 | Sissel Rønning

Date Mon 18 Nov
Time 10:30 11:30
Location Department of Bioscience, Ny Munkegade 114, 8000 Aarhus C, building 1540-116

Speaker: Natalie Elia,  Department of Life Sciences Ben Gurion University of the Negev Beer Sheva, Israel

ABSTRACT

Conserved from archaea to mammals, the ESCRT III filamentous system executes membrane constriction and fission in a variety of processes in cells. Yet, the mechanism and regulation of ESCRT-driven membrane fission in intact cells is still unresolved. In cytokinetic abscission of mammalian cells, a 1micron wide membrane bridge is constricted and cut within approximately 20 minutes.

The large-scale, ESCRT III-dependent, membrane constriction that occurs during abscission, offers an attractive model system to visualize ESCRTs in physiological context. We employ a variety of high resolution imaging techniques to study the ultrastructural organization of the ESCRT complex during abscission. Using data from Soft-X-ray tomography and 3D STORM we resolve structural evolution of the ESCRT-III complex during membrane constriction and fission in cells at nanoscale resolution. With this information in hand, we aim to unlock the mechanism of this ancient molecular machine.  

Everybody is welcome!

Microbiology, Department of Bioscience, Public / media, Staff